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The Animal Welfare Act is the principal Federal statute governing the sale, handling, transport, and use of animals in research, testing, and teaching. The United States Department of Agriculture, Animal and Plant Health Inspection ServiceAnimal Care implements the AWA through the Animal Welfare Regulations found in the Code of Federal Regulations, Title 9, Chapter 1, Subchapter A, Parts 1, 2, and 3.

The AWA and its amendments establish guidelines for the proper care and treatment of animals used in research, and for the organization and operation of institutional animal care and use committees. The law requires that the guidelines address humane care; minimization of pain and distress; consideration of alternatives; appropriate use of tranquilizers; analgesics; anesthetics; paralytics; and euthanasia; appropriate pre-surgical and post-surgical veterinary medical and nursing care for animals; psychological well-being of primates; and exercise for dogs. The requirements for reporting minority views of animal care committee members, for Animal Welfare Assurances, and for instruction or training in methods that limit the use of animals or limit animal distress, are all embodied in this Act. Compliance with the Animal Welfare Regulations, as applicable, is an absolute requirement of USAF policy.

Air Force Manual 40-401(I), “The Care and Use of Laboratory Animals in DoD Programs”
AFMAN 40-401(I) provides a complete description of DoD requirements for using animals.

Department of Defense Instruction 3216.01, “Use of Animals in DoD Programs”

Defense Federal Acquisition Regulation: Animal Welfare Clause (252.235-70002)

DoD Protocol Format (Full text)
This is the protocol format required for all intramural DoD laboratories.

The Guide for the Care and Use of Laboratory Animals (Free PDF download)

U.S. Government principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research, and Training (Full text)
These are the Principles which must be used by any government agency sponsoring research or teaching involving the use of laboratory animals.

AVMA Guidelines on Euthanasia (Full text PDF)
PHS policy requires that euthanasia methods be in accordance with this report unless scientifically justified.